Table 2.

Values of IC50 (micromolar) for proTWT, proTCC, proTY93A, and proTΔ154-167 for IgG aPS/PT and IgG aPT-A

VariableproTWTproTCCproTY93AproTΔ154-167Ratio (closed/open)
aPS/PT
 P771 ± 10>804.5 ± 0.84.5 ± 0.818
 P13>80>804.1 ± 1.13.5 ± 0.819
 P2422.4 ± 525 ± 53.2 ± 0.92.8 ± 0.88
aPT-A
 P72.6 ± 0.52.4 ± 0.52.7 ± 0.41.2 ± 0.30.9
 P133.8 ± 1.04.8 ± 1.13.7 ± 0.53.5 ± 0.51.3
 P241.7 ± 0.31.5 ± 0.52.8 ± 0.32.5 ± 0.80.5
  • Each titration was repeated twice, and the values of IC50 were determined by global fit using a simple binding equation (see "Materials and methods"). The ratio was calculated by divining the IC50 values of proTCC (closed) and proTY93A (open).