Table 1.

JAK2V617F allele burden in TNF-α+ monocytes

Patient4 h10 h
TNF-αTNF-α+TNF-αTNF-α+
19292.2157.5285.5882.65
22863.6657.9290.0178.10
23234.8842.6653.1351.90
25294.8490.5066.2063.45
25520.9576.8675.2480.23
  • MPN monocytes (n = 5) were stimulated with 10 ng/mL LPS for 4 or 10 hours in the presence of brefeldin A, and intracellularly stained for TNF-α. TNF-α+ cells were sorted and JAK2V617F allele burden was determined by quantitative PCR.