Table 1.

Enrichr analysis of scRNAseq data

Gene fractionEnriched terms*Adjusted PSource
AProcesses/pathways
 Top 200 expressed genes (all cells)Hemoglobin’s chaperone_Homo sapiens_h_ahspPathway6.46E−10BioCarta 2016
Heme biosynthesis_Homo sapiens_PWY-59201.82E−03HumanCyc 2016
Heme biosynthesis_Homo sapiens_P027461.39E−01Panther 2016
Hypoxic and oxygen homeostasis regulation of HIF-1-alpha_Homo sapiens_4c0f3584-6193-11e5-8ac5-06603eb7f3035.54E−02NCI-Nature 2016
Ribosome_Homo sapiens_hsa030107.22E−41KEGG 2016
HIF-1 signaling pathway_Homo sapiens_hsa040662.75E−02KEGG 2016
 Upregulated in GFP+Hemoglobin’s chaperone_Homo sapiens_h_ahspPathway8.26E−07BioCarta 2016
Heme biosynthesis_Homo sapiens_PWY-59204.54E−02HumanCyc 2016
Heme biosynthesis_Homo sapiens_P027467.41E−02Panther 2016
Ubiquitin protein ligase binding (GO:0031625)4.20E−02GO molecular function 2017
Oxygen transport (GO:0015671)1.29E−04GO biological process 2015
Oxidative stress_Homo sapiens_WP4081.12E−02WikiPathways 2016
Cellular responses to stress_Homo sapiens_R-HSA-22627522.82E−04Reactome 2016
HIF-1-α transcription factor network_Homo sapiens_20ef2b81-6193-11e5-8ac5-06603eb7f3035.57E−04NCI-Nature 2016
 Downregulated in GFP+Ribosome_Homo sapiens_hsa030104.75E−29KEGG 2016
tRNA binding (GO:0000049)6.01E−16GO molecular function 2017b
Peptide chain elongation_Homo sapiens_R-HSA-1569021.52E−31Reactome 2016
Eukaryotic Translation Elongation_Homo sapiens_R-HSA-1568421.71E−31Reactome 2016
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)_Homo sapiens_R-HSA-9759561.71E−31Reactome 2016
3′ -UTR-mediated translational regulation_Homo sapiens_R-HSA-1572795.82E−30Reactome 2016
BCell type
 Top 200 expressed genes (all cells)CD71+_EarlyErythroid1.90E−23Human Gene Atlas
Bone marrow (bulk tissue)2.98E−25ARCHS4 Tissues
Cord blood1.60E−23ARCHS4 Tissues
Erythroblast1.07E−03ARCHS4 Tissues
 Upregulated in GFP+CD71+_EarlyErythroid4.25E−49Human Gene Atlas
Erythroid_cell6.60E−30Jensen Tissues
Blood7.61E−13Jensen Tissues
Erythroblast1.96E−12ARCHS4 Tissues
CTranscription factor targets
 Upregulated in GFP+KLF15.196E−32Enrichr Submissions TF-Gene Coocurrence
KLF1_20508144_ChIP-Seq_FETAL-LIVER-ERYTHROID_Mouse1.490E−06ChEA 2016
GATA1_CHEA4.279E−15ENCODE and ChEA
GATA1_erythroblast_hg197.311E−9Consensus TFs from ChIP-X
GATA1_19941826_ChIP-Seq_K562_Human4.508E−8ChEA 2016
TAL1_20566737_ChIP-Seq_PRIMARY_FETAL_LIVER_ ERYTHROID_Mouse5.805E−12ChEA 2016
TAL1_erythroblast_mm94.769E−10ENCODE TF ChIP-seq 2015
  • * Enriched in different fractions of day-29 β_GFP reporter hiPSC-derived erythroid cells according to Enrichr analysis of scRNAseq data.

  • Gene set libraries were accessed through the Enrichr platform (http://amp.pharm.mssm.edu/Enrichr/).