Table 2.

Summary of affinity and kinetic values for E-Ig binding to E-selLs: CD34, CD44, CD43, and PSGL-1 using a SPR-based assay

CD34CD44
1st2nd3rdMean ± SEM1st2nd3rdMean ± SEM
KD, nM145268297236.7 ± 38234252214233 ± 9
koff-apparent, s−12.90E−041.60E−043.31E−042.6E−4 ± 0.4E−41.10E−042.00E−042.73E−041.9E−4 ± 0.4E−4
kon-apparent, M−1 s−1203059710541230 ± 3466417941280903 ± 156
CD43PSGL-1
1st2ndMean ± SEM1st2nd3rdMean ± SEM
KD, nM508.7376442 ± 47342.5207229259 ± 34
koff-apparent, s−14.80E−044.20E−044.5E−4 ± 0.2E−51.50E-041.65E−042.62E−041.9E−4 ± 0.3E−4
kon-apparent, M−1 s−19441063.81004 ± 434387971144793 ± 166