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Fluorescent labeling of CRISPR/Cas9 RNP for gene knockout in HSPCs and iPSCs reveals an essential role for GADD45b in stress response

Masoud Nasri, Perihan Mir, Benjamin Dannenmann, Diana Amend, Tessa Skroblyn, Yun Xu, Klaus Schulze-Osthoff, Maksim Klimiankou, Karl Welte and Julia Skokowa

Article Information

Citation 
vol. 3 no. 1 63-71
PubMed 

Print ISSN 
Online ISSN 
History 
  • Submitted December 24, 2017
  • Accepted November 28, 2018
  • Published online January 8, 2019.


Contributors 
  • Masoud Nasri, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;
  • Perihan Mir, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;2German Cancer Research Center, Heidelberg, Germany;
  • Benjamin Dannenmann, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;
  • Diana Amend, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;
  • Tessa Skroblyn, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;3Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany;
  • Yun Xu, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;
  • Klaus Schulze-Osthoff, 2German Cancer Research Center, Heidelberg, Germany;4Interfaculty Institute of Biochemistry, Tübingen University, Tübingen, Germany; and
  • Maksim Klimiankou, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;
  • Karl Welte, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;5University Children’s Hospital Tübingen, Tübingen, Germany
  • Julia Skokowa, 1Department of Oncology, Hematology, Immunology, Rheumatology, and Pulmonology, University Hospital Tübingen, Tübingen, Germany;2German Cancer Research Center, Heidelberg, Germany;

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